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KMID : 0861120090130020001
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Korean Journal of Oriental Preventive Medicine
2009 Volume.13 No. 2 p.1 ~ p.18
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Cytoprotective Effect of Phenolic Compounds Against Hexavalent Chromium-Induced Cytotoxicity
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Han Du-Seok
Kang Jeong-Il
Baek Seung-Hwa
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Abstract
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Objectives: In order to evaluate the cytotoxicity of hexavalent chromium, the cytoprotective effect of phenolic compounds against hexavalent chromium-induced cytotoxicity, cell viability, cell adhesion ability, lactate dehydrogenase(LDH) activity, and morphological changes of cells were examined.
Methods: We measured the cytotoxicity of hexavalent chromium with 3-[4,5-dimethyl-thiazol-2-yl]-2,5- diphenyltetrazolium bromide(MTT), 2,3-bis-[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-caboxanilide (XTT), LDH and DPPH methods.
Results: The cytotoxicity of hexavalent chromium(IC50, 44.0-51.0μM) was high according to the toxic criteria. Cytoprotective effect of phenolic compounds against IC50 value of hexavalent chromium in cell morphology increased in a concentration-dependent manner.
Conclusions: These results suggest that 3,4,5-trihydroxybenzoic acid may be used as a cytoprotective agent against chromium(IV)-mediated cytotoxicity.
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KEYWORD
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Cytoprotective effect, Hexavalent chromium, Chromium-mediated cytotoxicity, Cell viability, Cell adhesion ability
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